Product Description

IL18BP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6312-50, BS6312-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O95998

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:2000
IHC/IF: 1:50~1:200

Background: IL-18 (also referred to as IL-1γ) has been shown to augment the secretion of IFN-γ from T lymphocytes and to increase NK cell activity in spleen cells. IL-18 exhibits 19% and 12% identity with IL-1α and IL-β, respectively, over the twelve β-strands of the β-trefoil fold domain, which is a signature feature of the IL-1 family. The unusual leader sequence of IL-18 may be analogous to the IL-1β pro-domain, which must be cleaved by the serine protease ICE for optimal secretion and biological activity. IL-18, which was originally described as IGIF (IFN-γ-inducing factor), IL-18 is induced in mouse liver subsequent to challenge with lipopolysaccharide (LPS). IL-18 binding protein (IL-18BP) functions as an inhibitor of the early Th1 response by binding to IL-18 and inhibiting IFN-γ production. IL-18BP is a member of the immunoglobulin superfamily and shares some homology to IL-1RII.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IL18BP polyclonal antibody detects endogenous levels of IL18BP protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using IL18BP antibody., Immunohistochemistry of paraffin-embedded mouse heart using IL18BP antibody at dilution of 1:100 (40x lens)., Immunofluorescence analysis of A549 cell using IL18BP antibody. Blue: DAPI for nuclear staining.

Alternative Name: Interleukin-18-binding protein; IL-18BP; Tadekinig-alfa; IL18BP

Immunogen: Recombinant full length Human IL18BP.

Conjugate: Unconjugated

Modification: Unmodified