IL2 Polyclonal Antibody-BS60299

IL2 Polyclonal Antibody

Catalogue Numbers: BS60299-50, BS60299-100

Sizes: 50µl, 100µl

Alternative Name: Interleukin-2; IL-2; T-cell growth factor; TCGF; INN=Aldesleukin; IL2

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P60568

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Lymphokines are a group of signaling molecules involved in communication between cells of the immune system. Lymphokines secreted by activated lymphocytes include proteins such as IL-2. This protein is secreted primarily by helper T cells that have been activated through the T cell receptor complex or by other mitogens. Cells targeted by IL-2 include activated T helper and cytotoxic T cells, inducing their proliferation. The secretion of IL-2 can also act as a growth factor for B cells. To date, three different IL-2-dependent signal transduction pathways have been identified: the c-Fos/c-Jun induction pathway mediated by Src family protein-tyrosine kinases; the c-Myc induction pathway; and the Rapamycin-sensitive pathway; all of which result in the induction of Bcl-2. In addition, the transcription factor NFAT has been shown to play a major role in the regulation of IL-2 transcription and correlates to an age-related decline in the expression of IL-2.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IL2 polyclonal antibody detects endogenous levels of IL2 protein.

Bioworld Molecular Weight: ~ 18 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IL2 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: NIH-3T3 whole cell lysate
Lane 3: H9C2 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human IL2.

Conjugate: Unconjugated

Modification: Unmodified