IL2RA Polyclonal Antibody
Catalogue Numbers: BS60896-50, BS60896-100
Sizes: 50µl, 100µl
Alternative Name: Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25; IL2RA
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01589
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The IL-2 receptor is amulticomponentcomplexconsisting of three subunits,α,
β and γ, each ofwhich isrequired for high-affinity binding of IL-2. Theαchain
functions primarilyin binding IL-2, whereasthe β and γ chainscontribute to IL-2 binding and are essential to IL-2-induced activation ofsignaling pathways leading to T cell growth. Both IL-4R and IL-7R were initially described as single chain, high-affinityligand-binding cytokine receptors. However, it is now well established that the IL-2Rγ chain functions as a second subunit of the high affinityIL-4R and IL-7R receptors. Consequently, the originally described subunits of these latter receptors are nowreferred to asIL-4Rαand IL-7Rα, respectively,while the common subunit isreferred to as γc. Although the common γ chain enhancesligand binding in these three cytokine receptors, it has no capacityto bind these ligands on its own. There is evidence that the γcchain is also a subunit of IL-13R.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: IL2RA polyclonal antibody detects endogenous levels of IL2RA protein.
Bioworld Molecular Weight: ~ 55 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of IL2RA polyclonal antibody at 1:500 dilution
Lane 1: Thp-1 whole cell lysate
Lane 2: H9C2 whole cell lysate
Lane 3: Raw264.7 whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human IL2RA.
Conjugate: Unconjugated
Modification: Unmodified