Product Description

IL3RA Polyclonal Antibody

Catalogue Numbers: BS60620-50, BS60620-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Swiss-Prot: P26951

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: IL3RA/CD123 is the alpha subunit of an IL-3 receptor that also contains the common beta chain (βc) shared with receptors for other cytokines. IL3RA/CD123 alone binds to IL-3 with high specificity but low affinity and it does not transduce intracellular signaling, while βc alone does not bind to IL-3 appreciably. Together, IL3RA/CD123 and βc form the functional high-affinity receptor for IL-3. IL3RA/CD123 is mainly expressed on the hematopoietic progenitor cells, primarily the granulocytic and monocytic linages . IL3RA/CD123 is highly expressed on leukemic stem cells in the majority of AML (acute myeloid leukemia) patients and is correlated with poor prognosis. In addition, overexpression of IL3RA/CD123 has also been reported for other hematopoietic malignancies. It is considered as a valuable therapeutic target for these malignancies.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IL3RA polyclonal antibody detects endogenous levels of IL3RA protein.

Extra Notes: Western blot (WB) analysis of IL3RA polyclonal antibody at 1:1000 dilution
Lane1:CT-26 whole cell lysate(30ug)
Lane2:C6 whole cell lysate(30ug)
Lane3:HepG2 whole cell lysate(30ug)
Lane4:HCT116 whole cell lysate(30ug)
Lane5:A549 whole cell lysate(30ug)

Bioworld Molecular Weight: ~ 60 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; CD_antigen: CD123

Immunogen: Synthetic peptide, corresponding to Human IL3RA.

Conjugate: Unconjugated

Modification: Unmodified