Product Description

IL6 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB9296-50, MB9296-100

Product: Mouse IgG2b, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P05231(Human) P08505(Mouse) P20607(Rat)

Host: Mouse

Reactivity: Human, Mouse, Rat

Applications: WB, IHC ICC

All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200

Background: Interleukin-6, or IL-6, is a multifunctional protein, 212 amino acids in length, that plays critical roles in host defense, immune response and hematopoiesis. IL-6 is constitutively expressed by epidermal Langerhans cells and its expression is induced in stimulated keratinocytes. IL-6, IL-1β and TNFα act as endogenous pyrogens, regulating the fever response to bacterial invasion. The IL-6 receptor is a trimeric complex composed of an IL-6-specific α chain and a homodimer of the gp130 glycoprotein common to the IL-6, IL-11, CNTF, OSM and LIF receptors. Stimulation with IL-6 leads to gp130 homodimerization and the activation of associated kinases JAK1 and JAK2. Once activated, JAK1 and JAK2 phosphorylate Stat3, causing its nuclear translocation and transcription of Stat3-responsive genes. IL-6 has also been shown to activate the Ras/MAP kinase pathway, which regulates NFIL6 transcription.

Purification and Purity: ProG affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: IL6 monoclonal antibody detects endogenous levels of IL6 protein.

Bioworld Molecular Weight: 24 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of IL6 on recombinant protein lysate using anti-IL6 antibody at 1/1,000 dilution., ICC staining IL6 (green) in PC-3M cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: IL-6, BSF-2, CDF, IFN-beta-2, IL6, IFNB2, Interleukin BSF 2, B cell differentiation factor, B cell stimulatory factor 2, B-cell stimulatory factor 2, BSF 2, BSF-2, BSF2, CDF, CTL differentiation factor, Hepatocyte stimulatory factor, HGF, HSF, Hybridoma growth factor, Hybridoma growth factor Interferon beta-2, IFN-beta-2, IFNB2, IL 6, IL-6, IL6, IL6, Interferon beta 2, Interferon beta-2, Interleukin 6, Interleukin 6 (interferon beta 2), Interleukin BSF 2, Interleukin-6,

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified