ILF3 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90711-50, BS90711-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q12906(Human) Q9Z1X4(Mouse) Q9JIL3(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, IP, ICC IF, IHC FC
All Applications: WB:1:500-1:2,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
IP:1:10-1:50
Background: ILF3 (interleukin enhancer binding factor 3) or TCP80 (translational control protein 80), is a ubiquitously expressed nuclear protein that exists in a heterodimer with NF45. NF90 contains two DRBM (double-stranded RNA(dsRNA-)-binding) domains and one DZF domain and, in association with NF45 primarily participates in the regulation of IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES). NF45 and NF90 belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. Due to alternative splicing events, six isoforms exist for NF90, namely NFAR-2 (or ILF3-E), NFAR-1 (or DRBP76), isoform 3, DRBP76α (or ILF3-A), DRBP76δ (also known as DRBP76γ or ILF3-C) and isoform 6.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: ILF3 polyclonal antibody detects endogenous levels of ILF3 protein.
Bioworld Molecular Weight: 75/95 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of ILF3 on rat heart tissue (1) and 293 cell (2) lysate using anti-ILF3 antibody at 1/1,000 dilution., ICC staining ILF3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: DRBP76, MPP4, NFAR, NF-AT-90, TCP80, ILF3, CBTF, Double stranded RNA binding protein 76, Double-stranded RNA-binding protein 76, DRBF, DRBP76, ILF3, ILF3, Interleukin enhancer binding factor 3, Interleukin enhancer-binding factor 3, M phase phosphoprotein 4, M-phase phosphoprotein 4, MPHOSPH4, MPP4, NF AT 90, NF-AT-90, NF110, NF90, NFAR, Nuclear factor associated with dsRNA, Nuclear factor of activated T cells 90 kDa, Nuclear factor of activated T-cells 90 kDa, TCP80, Translational control protein 80,
Immunogen: Recombinant protein of N-terminal human ILF3.
Conjugate: Unconjugated
Modification: Unmodified