Product Description

INCENP Polyclonal Antibody

Catalogue Numbers: BS60432-50, BS60432-100

Sizes: 50µl, 100µl

Alternative Name: Inner centromere protein

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NQS7

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: A replicated chromosome includes two kinetochores that control chromosome segregation during mitosis. The centromere proteins CENP-A, CENP-B, CENP-C, CENP-E, CENP-F (also designated mitosin), CENP-H and INCENP are kinetochore proteins that are involved in mitotic events. The centromere proteins are expressed at different levels throughout the cell cycle and are involved in the formation of the centromere and the organization and function of the kinetochore. INCENP, which also is designated inner centromere protein, is a chromosomal passenger protein that is crucial for chromosome segregation. During mitosis it is also required for cytokinesis onset. This protein, which can form a homodimer or a heterodimer, binds directly to microtubules and interacts with AURKB, AURKC, CBX3 and β Tubulin. This nuclear protein localizes to the mitotic spindle, methaphase chromosomes and during anaphase, to the equatorial cortex.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: INCENP polyclonal antibody detects endogenous levels of INCENP protein.

Bioworld Molecular Weight: ~ 140 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of INCENP polyclonal antibody at 1:500 dilution
Lane 1: HEK293T cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: PC12 cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human INCENP.

Conjugate: Unconjugated

Modification: Unmodified