INCENP Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A0622-20, A0622-100
Citations, Manuals and MSDS Available upon request.
Background: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger, ' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143) ; MCAK (MIM 604538) ; KID (MIM 603213) ; cytoplasmic dynein (e.g., MIM 600112) ; CliPs (e.g., MIM 179838) ; and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Rat
Protein Weight: 105kDa
Observed Molecular Weight: 120-140kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 3619
SWISS: Q9NQS7
Alternate Names: INCENP
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Cell Biology Developmental Biology, Cell Cycle.
NCBI Alias: INCENP
Research Use Only