Product Description

iNOS (Phospho-Y151) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64565-50, BS64565-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35228

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB:1:500~1:1000 IHC:1:50~1:200

Background: Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: iNOS (Phospho-Y151) polyclonal antibody detects endogenous levels of iNOS protein only when phosphorylated at Tyr151.

Bioworld Molecular Weight: ~ 130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western Blot (WB) analysis of iNOS (Phospho-Y151) polyclonal antibody at 1:500 dilution
Lane A: Hela whole cell lysate
Lane B: Jurkat whole cell lysate
Lane C: MCF-7 whole cell lysate

Alternative Name: Nitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; Short name=iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase; NOS 2; NOS2A; Inos

Immunogen: Synthetic phosphopeptide derived from human iNOS around the phosphorylation site of Tyrosine 151.

Conjugate: Unconjugated

Modification: Phosphorylation