Product Description

INPP5J Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS8311-50, BS8311-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q15735

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:10 - 1:100

Background: Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 107kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using INPP5J antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of U2OS cells using INPP5J antibody. Blue: DAPI for nuclear staining.

Alternative Name: INPP5J; INPP5; PIB5PA; PIPP

Immunogen: Recombinant fusion protein of human INPP5J(NP_001002837.1).

Conjugate: Unconjugated

Modification: Unmodified