Instant ELISA Kit for Mucin 5 Subtype AC (MUC5AC)
Sizes: 24T, 48T, 96T
Catalogue Numbers: IEA756Mu-24, IEA756Mu-48, IEA756Mu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: Instant kit
Target: Mucin 5 Subtype AC
Alternative Names: TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5, Subtypes A And C, Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein
Target Species: Mouse
Uniprot: E9PWB6
Gene ID: 17833
Featured Series Function: Detects protein (quick procedure in 1 hrs)
Specificity: Reactive with Mouse MUC5AC / Mucin 5 Subtype AC
Method: Colormetric
Detection principle: Double-antibody Sandwich
Detection
range: 0.156-10ng/mL
Sensitivity: 0.055ng/mL
Assay Time: 1h, 10min
Sample Size: 100uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Instant Mucin 5 Subtype AC (MUC5AC).
No significant cross-reactivity or interference between Instant Mucin 5 Subtype AC (MUC5AC) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Mucin 5 Subtype AC (MUC5AC) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Mucin 5 Subtype AC (MUC5AC) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 50μL standard or sample and 50μL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50μL Stop Solution. Read at 450nm immediately.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Instant Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Instant Mucin 5 Subtype AC (MUC5AC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Mucin 5 Subtype AC (MUC5AC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Tumor immunity; Infection immunity; Endocrinology; Pulmonology;
Research Use Only