Insulin Polyclonal Antibody-BS6890

Insulin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6890-50, BS6890-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P01308

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 6KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using Insulin antibody at 1:671 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb at dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of human pancreas cells using Insulin Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse pancreas cells using Insulin Rabbit pAb at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: IDDM; IDDM1; IDDM2; ILPR; IRDN; MODY10; INS; Insulin; insulin

Immunogen: Recombinant fusion protein of human INS(NP_000198.1).

Conjugate: Unconjugated

Modification: Unmodified