Product Description

Integrin αV Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90723-50, BS90723-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06756(Human) P18564(Human) P43406(Mouse) Q9Z0T9(Mouse) Q6AYF4(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, FC

All Applications: WB:1:1,000-1:2,000
FC:1:50-1:100

Background: Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, collagen and vitronectin. Certain integrins can also bind to soluble ligands, such as fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: Integrin αV polyclonal antibody detects endogenous levels of Integrin αV protein.

Bioworld Molecular Weight: 86/116 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Integrin alpha V+beta 6 on different lysates using anti-Integrin alpha V+beta 6 antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549
Lane 2: PC-12, Flow cytometric analysis of MCF-7 cells with Integrin alpha V antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Alternative Name: ITGAV, MSK8, VNRA, ITGB6, CD51, Integrin alpha-V light chain, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), Integrin, alpha V, Integrin, beta 6, ITAV, ITGAV, ITGB6, Vitronectin receptor subunit alpha,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified