Product Description

Integrin alpha 6 Monoclonal Antibody

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Sizes: 50µl, 100µl

Catalogue Numbers: BS65510-50, BS65510-100

Swiss-Prot: P23229

Host: Rabbit

Integrin alpha 6 Monoclonal Antibody-BS65510

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC-P

All Applications: WB=1:500-2000 IHC-P=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

Background: Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis.

Purification and Purity: affinity purified by Protein A

Specificity: Integrin alpha 6 monoclonal Antibody detects endogenous levels of Integrin alpha 6 protein.

Bioworld Molecular Weight: ~120kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Anti-Integrin alpha 6 at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (human tonsil)Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 6) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C.

Alternative Name: Integrin alpha-6; Alpha6p; VLA 6 alpha subunit; VLA 6; VLA-6; Integrin α6; CD49 antigen-like family member F; CD49f; Integrin alpha6B; Integrin, alpha 6; ITA6_HUMAN; ITGA6; ITGA6B; Processed integrin alpha-6.

Immunogen: Recombinant Human Integrin alpha 6:1-100/1130

Modification: Unmodified

Conjugate: Unconjugated