Product Description

IP3KA (H431) Polyclonal Antibody

Catalogue Numbers: BS1959-50, BS1959-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P23677

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) regulates the level of calcium within the cell by releasing calcium from intracellular stores. Ins(1,4,5)P3 is phosphorylated by inositol 1,4,5-trisphosphate 3-kinase (IP3K) to form inositol 1,3,4,5-tetrakisphosphate (Ins(1,4,5)P4), which is is thought to regulate the influx of calcium across the plasma membrane. IP3K exists as three isoforms, IP3KA, B, and C. IP3KA, the most highly characterized isoform, is approximately 51 kDa and is expressed in rat brain and testis. IP3KB has a molecular weight of 74 kDa and is expressed in various rat tissues such as lung, thymus, testis, brain, and heart. IP3K activity is stimulated in the presence of calmodulin via phosphorylation by cAMP-dependent protein kinase, protein kinase C, or calcium/calmodulin dependent protein kinase II and, subsequently, mediates the inositol phosphate signaling pathways.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IP3KA (H431) polyclonal antibody detects endogenous levels of IP3KA protein.

Extra Notes: Western blot (WB) analysis of IP3KA (H431) polyclonal antibody at 1:500 dilution
Lane1:THP-1 whole cell lysate(40ug)
Lane2:The Lung tissue lysate of Rat(40ug)
Lane3:The Lung tissue lysate of Mouse(40ug)
Lane4:Jurkat whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 51 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: 1D myo inositol trisphosphate 3 kinase A; EC 2.7.1.127; Inositol 1 4 5 trisphosphate 3 kinase A; Inositol trisphosphate 3 kinase A; IP3 3 kinase A; IP3K A; IP3KA;

Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human IP3KA.

Conjugate: Unconjugated

Modification: Unmodified