Product Description

IPP2 (Q116) Polyclonal Antibody

Catalogue Numbers: AP0459-50, AP0459-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P41236

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:1000~1:2000

Background: Two inhibitors of protein phosphatase 1 (PP1) include the phosphatase inhibitor 1 (IPP-1) and phosphatase inhibitor 2 (IPP-2) . IPP-2, also known as I-2, interacts with the catalytic subunit of PP1 to form the heterodimer PP1I. The PP1I complex is present in the cytosol of cells in a broad range of vertebrate and invertebrate species. Although the heterodimer itself is inactive, a reversible phosphorylation of IPP-2 at Thr 72 by glycogen-synthase-kinase (GSK3) initiates activation of the heterodimer complex in vitro. Phosphorylation of IPP-2 by casein kinase-II at Ser 86, Ser 120, and Ser 121 enhances the rate of phosphorylation by GSK3 at Thr 72 and effectively activates the heterodimer complex. Besides moderating PP1 activity, IPP-2 may play a role as a chaperone for the correct folding of PP1.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 107% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IPP2(Q116) polyclonal antibody detects endogenous levels of IPP2 protein.

Extra Notes: Western blot (WB) analysis of IPP2 (Q116) pAb at 1:2000 dilution
Lane1:EC9706 whole cell lysate(40ug)
Lane2:HCT116 whole cell lysate(40ug)
Lane3:AML-12 whole cell lysate(40ug)
Lane4:H9C2 whole cell lysate(40ug)

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Protein phosphatase inhibitor 2; IPP-2; PPP1R2; IPP2

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human IPP2.

Conjugate: Unconjugated

Modification: Unmodified