Product Description

IQGAP1 Polyclonal Antibody

Catalogue Numbers: BS5767-50, BS5767-100

Sizes: 50µl, 100µl

Alternative Name: Ras GTPase-activating-like protein IQGAP1; p195; KIAA0051

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P46940

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IQGAP1 polyclonal antibody detects endogenous levels of IQGAP1 protein.

Bioworld Molecular Weight: ~ 190 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IQGAP1 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: sp2/0 cell lysate
Lane 3: H9C2 cell lysate

Immunogen: Synthetic peptide, corresponding to amino acids 291-336 of Human IQGAP1.

Conjugate: Unconjugated

Modification: Unmodified