IQGAP1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90734-50, BS90734-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P46940(Human) Q9JKF1(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC, IF, IHC, FC
All Applications: WB:1:500-1:2,000
ICC/IF:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100
Background: IQGAP1, for IQ motif containing GTPase activating protein, is a Ras GAP-related, Actin-binding protein that interacts with the small GTPases Cdc42 and Rac1. The C-terminus of IQGAP1 is essential for interacting with Cdc42 and, in addition, IQGAP1 contains a WW domain and a predicted N-terminal coiled-coil region, which may be involved in IQGAP dimerization. Expression of IQGAP1 is highest in placenta, lung and kidney, where it co-localizes with Cdc42 to the cytoskeleton and assists with Cdc42 in mediating the regulation of cell proliferation, polarity and cell morphology. IQGAP1 regulates cadherin-mediated cell adhesion via binding to E-cadherin, b-catenin and a-catenin. This association induces the accumulation of these proteins at the site of cell-cell contact. IQGAP1 is negatively regulated by calmodulin, which binds to IQGAP1 in a calcium-dependent manner and disrupts IQGAP1 from associating with Cdc42.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Specificity: IQGAP1 polyclonal antibody detects endogenous levels of IQGAP1 protein.
Bioworld Molecular Weight: 189 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of IQGAP1 on different lysates using anti-IQGAP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HUVEC
Lane 2: A431
Lane 3: Mouse placenta, ICC staining IQGAP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: IQGAP1, KIAA0051, HUMORFA01, IQ motif containing GTPase activating protein 1, IQGA1, Iqgap1, KIAA0051, p195, Ras GTPase activating like protein 1, Ras GTPase-activating-like protein IQGAP1, RasGAP-like with IQ motifs, SAR1,
Immunogen: Recombinant protein corresponding to N-terminal human IQGAP1.
Conjugate: Unconjugated
Modification: Unmodified