IRF-2 (K129) Polyclonal Antibody-BS1930

IRF-2 (K129) Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: BS1930-50, BS1930-100

Sizes: 50µl, 100µl

Swiss-Prot: P14316

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

Background: Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3γ p48 (the 48 kDa component of the ISGF-3 complex) and IFN consensus sequence-binding protein (ICSBP).

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IRF-2 (K129) polyclonal antibody detects endogenous levels of IRF-2 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IRF-2 (K129) pAb at 1:500 dilution
Lane 1: A549 whole cell lysate (40ug)
Lane 2: SGC7901 whole cell lysate (40ug)
Lane 3: HepG2 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)
Lane 5: AML-12 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of IRF-2 (K129) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 47 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: IRF-2; IRF2; Interferon regulatory factor 2

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human IRF-2.

Conjugate: Unconjugated

Modification: Unmodified