Product Description

IRF-3 (A379) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS1542-50, BS1542-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimμlated cells, IRF-3 is present in the cytoplasm. Viral infection resμlts in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal Ser and Thr residues (between 385 and 405), leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity. During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and a kinase complex that includes IKKε and TBK1. IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double-stranded RNA treatment. These events likely play a role in activation of IRF-3.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC, IF

Alternate Names: IRF3, IRF-3, IRF3, Interferon regμlatory factor 3

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Synthetic peptide, corresponding to amino acids 346-397 of Human IRF-3.

Dilution: IHC: 1:50~1:200
IF: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IRF-3 (A379) polyclonal antibody detects endogenous levels of IRF-3 protein.

Molecular Weight: ~ 47, 55 kDa

Pathway: Immune Cell Signaling, STING Signaling, Toll-like Receptor signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q14653

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only