IRF2 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90737-50, BS90737-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P14316(Human) P23906(Mouse)
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, ICC/IF
All Applications: WB:1:1,000-1:2,000
ICC:1:50-1:200
Background: Interferon regulatory factor-1 (IRF-1) and IRF-2 have been identified as novel DNA-binding factors that function as regulators of both type I interferon (interferon-α and β) and interferon-inducible genes. The two factors are structurally related, particularly in their N-terminal regions, which confer DNA binding specificity. In addition, both bind to the same sequence within the promoters of interferon-α and interferon-β genes. IRF-1 functions as an activator of interferon transcription, while IRF-2 binds to the same cis elements and represses IRF-1 action. IRF-1 and IRF-2 have been reported to act in a mutually antagonistic manner in regulating cell growth; overexpression of the repressor IRF-2 leads to cell transformation while concomitant overexpression of IRF-1 causes reversion. IRF-1 and IRF-2 are members of a larger family of DNA binding proteins that includes IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, ISGF-3? p48 (a component of the ISGF-3 complex) and IFN consensus sequence-binding protein (ICSBP).
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: IRF2 polyclonal antibody detects endogenous levels of IRF2 protein.
Bioworld Molecular Weight: 50 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of IRF2 on human lung lysates using anti-IRF2 antibody at 1/1,000 dilution., ICC staining IRF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: IRF-2, IRF2, DKFZp686F0244, Interferon regulatory factor 2, IRF 2, IRF-2, IRF2, IRF2,
Immunogen: recombinant protein
Conjugate: Unconjugated
Modification: Unmodified