Irf3 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A24864-20, A24864-100
Citations, Manuals and MSDS Available upon request.
Background: Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
Category: Polyclonal Antibodies
Applications: WB, IHC-P, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 46kDa
Observed Molecular Weight: 47kDa
Immunogen: Recombinant protein (or fragment) .
Species: Mouse
GeneID: 54131
SWISS: P70671
Alternate Names: IRF-3, IIAE7, IRF 3
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:1000 IHC-P, 1:50 - 1:200 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Immunology, Innate Immunity, Cytokines, Interferons.
NCBI Alias: Irf3
Research Use Only