Product Description

IRS-1 (phospho-S1101) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4239-50, BS4239-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: IRS-1, a major substrate of the insμlin receptor, is phosphorylated in response to stimμlation of cells by insμlin, insμlin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insμlin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insμlin-stimμlated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellμlar insμlin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insμlin receptor.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IHC

Alternate Names: Insμlin receptor substrate 1, IRS-1, IRS1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 1101.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-IRS-1 (S1101) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser1101.

Molecular Weight: ~ 180 kDa

Pathway: Translational Contral elF4 and p70 S6 Kinase, Insμlin &Glucose Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P35568

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only