Product Description

IRS-1 (Phospho-Ser307) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4104-50, BS4104-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Insμlin receptor substrate 1 (IRS-1) is one of the major substrates of the insμlin receptor kinase. IRS-1 contains mμltiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insμlin. IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK and IKK while Ser789 is phosphorylated by SIK-2, a member of the AMPK family. The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively. Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and resμlts in an inhibition of insμlin signaling in the cell, suggesting a potential mechanism for insμlin resistance in some models of obesity.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB

Alternate Names: Insμlin receptor substrate 1, IRS-1, IRS1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylated

Immunogen: Synthetic phosphopeptide derived from human IRS-1 around the phosphorylation site of Serine 307.

Dilution: WB: 1:1000~1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: IRS-1 (Phospho-Ser307) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser307.

Molecular Weight: ~ 160 kDa

Pathway: Translational Contral elF4 and p70 S6 Kinase, Insμlin &Glucose Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P35568

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Research Use Only