Product Description

IRX3 Polyclonal Antibody

Catalogue Numbers: BS61032-50, BS61032-100

Sizes: 50µl, 100µl

Alternative Name: Iroquois-class homeodomain protein IRX-3; Homeodomain protein IRXB1; Iroquois homeobox protein 3; IRX3; IRXB1

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P78415

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:1000

Background: The Iroquois homeobox gene family of transcription factors regulate aspects of embryonic development including anterior/posterior and dorsal/ventral axis patterning in the central nervous system. The Iroquois family are clustered on two loci, IRXA and IRXB, which map to chromosomes 8 and 13 in mice. The IRXA group includes IRX, IRX2 and IRX4; the IRXB group comprises IRX3, IRX5 and IRX6. IRX1 and IRX2 are both widely expressed during development in the lung epithelium and also in the ventricular septum. IRX1 and IRX2 also play a role in digit formation (E11.5–E14.5). The IRX gene family members are each expressed in a distinct pattern during mouse heart development. Specifically, IRX1 and IRX2 are expressed in the ventricular septum and IRX3 is expressed in the ventricular trabeculated myocardium. In addition, IRX4 is expressed in the linear heart tube and the AV canal; IRX5 is expressed in the endocardium lining the ventricular and atrial myocardium. Furthermore, the IRX4 gene may modulate cardiac development and function. Although the heart of IRX4(-) mice appears to develop normally, adult IRX4(-) mice exhibit cardiomyopathy, including cardiac hypertrophy and decreased contractility.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IRX3 polyclonal antibody detects endogenous levels of IRX3 protein.

Bioworld Molecular Weight: ~ 52 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of IRX3 polyclonal antibody at 1:500 dilution
Lane 1: RAW264.7 whole cell lysate Lane 2: PC12 whole cell lysate
Lane 3: HELA whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human IRX3.

Conjugate: Unconjugated

Modification: Unmodified