Product Description

Islet-2 Polyclonal Antibody

Catalogue Numbers: BS62288-50, BS62288-100

Sizes: 50µl, 100µl

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96A47

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB IF

All Applications: WB:1:500~1:1000 IF:1:50~1:200

Background: Islet-2 (insulin gene enhancer protein ISL-2) is a 359 amino acid protein encoded by the human gene ISL2. Islet-2 is a nuclear protein that contains two N-terminal LIM domains, followed by a homeodomain and a serine/glutamine/ threonine-rich C-terminus. Islet-2 is a transcriptional factor that defines subclasses of motor neurons that segregate into columns in the spinal cord and select distinct axon pathways. Islet-1 and Islet-2 are initially expressed by all postmitotic spinal motor neurons prior to diversification of somatic and visceral neuronal fates. Somatic, but not visceral, motor neurons maintain Islet-2 expression at later embryonic stages. An early phase of Islet-2 expression by prospective visceral motor neurons of the sympathetic preganglionic motor column is critical for the emergence of complete visceral motor neuron character. Mutations that reduce or eliminate both Islet-1 and Islet-2 activity will result in pronounced defects in visceral motor neuron generation and eroded somatic motor neuron character.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Islet-2 polyclonal antibody detects endogenous levels of Islet-2 protein.

Extra Notes: Western blot (WB) analysis of Islet-2 polyclonal antibody at 1:500 dilution
LaneA:Hela whole cell lysate
LaneB:The Liver tissue lysate of Mouse
LaneC:The Liver tissue lysate of Rat

Bioworld Molecular Weight: ~ 40 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: Insulin gene enhancer protein ISL-2; Islet-2; ISL2

Immunogen: Synthetic peptide, corresponding to Human Islet-2.

Conjugate: Unconjugated

Modification: Unmodified