ITIH3 Polyclonal Antibody
Catalogue Numbers: BS60741-50, BS60741-100
Sizes: 50µl, 100µl
Alternative Name: Inter-alpha-trypsin inhibitor heavy chain H3; ITI heavy chain H3; ITI-HC3; Inter-alpha-inhibitor heavy chain 3; Serum-derived hyaluronan-associated protein; SHAP; ITIH3
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: Q06033
Host: Rabbit
Reactivity: Human
Applications: WB
All Applications: WB: 1:500~1:1000
Background: The inter-α trypsin inhibitor (ITI) family is a group of structurally related
plasma serine protease inhibitors synthesized in the liver and built up from different combinations of three highly homologous heavy chains (ITI-H1, ITI-H2 and ITI-H3) and one light chain (Bikunin). A fourth member of the ITI family, ITI-H4 (also known as I α IH4P) harbors a Pro-rich region (PRR) in its C-terminus. ITI is a glycoprotein composed of three polypeptides linked by chondroitin sulphate: two heavy chains, ITI-H1 and ITI-H2, and Bikunin. Bikunin confers the protease-inhibitor function of ITI. The heavy chains of the ITI family, designated as SHAPs (for serum-derived hyaluronan-associated proteins),bind covalently to hyaluronic acid (HA), resulting in pericellular matrix stabilization. Although ITI-H1, ITI-H3 and Bikunin have antitumoral and antimetastatic properties in the cell, these proteins are also associated with malignant transformation of lung tissue. ITI-H3 and Bikunin associate to form pre-α-trypsin inhibitor (PαI), a serine-proteinase inhibitor found in human serum.ITI-H3 mRNA levels increase in response to IL-6.
Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: ITIH3 polyclonal antibody detects endogenous levels of ITIH3 protein.
Bioworld Molecular Weight: ~ 100 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot (WB) analysis of ITIH3 polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Immunogen: A synthetic peptide corresponding to residues in Human ITIH3
Conjugate: Unconjugated
Modification: Unmodified