JNK1/2/3 (Phospho-T183) Rabbit Monoclonal Antibody -BS9939M

JNK1/2/3 (Phospho-T183) Rabbit Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS9939M-50, BS9939M-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P45983/P45984/P53779

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB: 1:1000-1:2000
IHC/ICC/IF: 1:50-1:200
FC: 1:50-1:100

Background: The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in Ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun and other signaling kinases, including MEKK1 and p38. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).

Purification and Purity: Protein A affinity purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This antibody detects endogenous levels of JNK1 protein only when phosphorylated at Thr183. This antibody also recognizes JNK2 and JNK3 when phosphorylated at the corresponding residues.

Bioworld Molecular Weight: ~ 46, 54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of JNK1/2/3 (Phospho-T183) Rabbit mAb at 1:1000 dilution
Lane 1: NIH/3T3 whole cell lysate treated with Anisomycin
Lane 2: NIH/3T3 whole cell lysate, Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3(T183) antibody. Counter stained with hematoxylin., ICC staining Phospho-JNK1/2/3(T183) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.

Alternative Name: JNK 46; JNK 55; JNK; JNK1; JNK1A2; JNK2; JNK21B1/2; JNK2ALPHA; JNK2B; JNK2BETA; JNK3; JNK3A; MAP kinase 8; MAP kinase 9; MAP kinase p49 3F12; MAPK 8; MAPK 9; MAPK10; MAPK8; MAPK9; MGC50974; Mitogen activated protein kinase 10; Mitogen activated protein kinase 8; Mitogen activated protein kinase 9;

Immunogen: Recombinant antibody.

Conjugate: Unconjugated

Modification: Phosphorylation