Product Description

JNK1/2/3 (Phospho-T183/Y185) Rabbit Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66418-50, MB66418-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase . Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimμlation of MEKKs and MLKs. Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimμlation of a germinal center kinase (GCK) family member. There are three SAPK/JNK genes each of which undergoes alternative splicing, resμlting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regμlate transcription through its effects on c-Jun, ATF-2, and other transcription factors.

Category: Primary Antibody

Reactivity: Human, Rat

Host: Rabbit

Applications: WB, IP

Alternate Names: JNK1, PRKM8, SAPK1, SAPK1C, Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: A synthetic phosphopeptide corresponding to residues surrounding Thr183/Tyr185 of human JNK1

Dilution: WB (1/500 - 1/1000), IP (1/10 - 1/50)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of JNK1/2/3 (pT183/Y185) protein.

Molecular Weight: ~ 46, 50 kDa

Pathway: Regμlation of Apoptosis, Death Receptor Signaling, ErbB HER Signaling, Inhibition of Apoptosis, Insμlin &Glucose Signaling, Mitochondrial Control of Apoptosis, NF-kB Signaling, SAPK JNK Signaling Cascades, T cell receptor signaling, TGFβ Smad signaling, Toll-like Receptor signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P45983

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Research Use Only