Product Description

JNK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90753-50, BS90753-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P45984(Human) Q9WTU6(Mouse) P49186(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC, IHC, IP, FC

All Applications: WB:1:1,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1, and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2, and JNK3β2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: JNK2 polyclonal antibody detects endogenous levels of JNK2 protein.

Bioworld Molecular Weight: 46/54 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of JNK2 on SHG-44 cell lysates using anti-JNK2 antibody at 1/1,000 dilution., ICC staining JNK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: MAP kinase 9, MAPK 9, SAPK1a, JNK2, PRKM9, SAPK1A, c Jun kinase 2, C Jun N terminal kinase 2, c-Jun N-terminal kinase 2, JNK 55, JNK-55, JNK2 alpha, JNK2, JNK2 beta, JNK2A, JNK2alpha, JNK2B, JNK2BETA, Jun kinase, MAP kinase 9, MAPK 9, Mapk9, Mitogen activated protein kinase 9, Mitogen-activated protein kinase 9, MK09, P54a, p54aSAPK, PRKM9, Protein kinase, mitogen-activated, 9, SAPK alpha, SAPK, SAPK1a, Stress activated protein kinase 1a, Stress-activated protein kinase JNK2,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified