JunD (Phospho-S255) Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS94014-50, BS94014-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P17535(Human) P15066(Mouse) P52909(Rat)
Host: Rabbit
Reactivity: Human, Mouse, Rat
Applications: WB, ICC/IF, IHC
All Applications: WB:1:1,000
ICC:1:50-1:200
IHC:1:50-1:200
Background: The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.
Purification and Purity: ProA affinity purified
Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity: JunD (Phospho-S255) polyclonal antibody detects endogenous levels of JunD protein only when phosphorylated at S255.
Bioworld Molecular Weight: 35 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: ICC staining Phospho-JunD(S255) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS., ICC staining Phospho-JunD(S255) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name: JUND, Activator protein 1, AP 1, AP1, Jun D, jun D proto oncogene, Jund, JunD FL isoform, JUND, Transcription factor jun D, Transcription factor jun-D,
Immunogen: Synthetic phospho-peptide corresponding to residues surrounding Ser255 of human JunD.
Conjugate: Unconjugated
Modification: Phosphorylation