Product Description

Kaiso/ZBTB33 Polyclonal Antibody

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Sizes: 50µl, 100µl

Catalogue Numbers: BS75054-50, BS75054-100

Swiss-Prot: Q86T24

Host: Rabbit

Kaiso/ZBTB33 Polyclonal Antibody-BS75054

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Reactivity: Human, Mouse

Applications: WB, IHC

All Applications: WB,1:1000 - 1:3000 | IHC,1:50 - 1:200

Background: This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-alpha2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Unmodification

Bioworld Molecular Weight: 74kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Kaiso/Kaiso/ZBTB33 antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded human liver using Kaiso/Kaiso/ZBTB33 Rabbit pAb at dilution of 1:350.Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: ZBTB33; ZNF-kaiso; ZNF348

Immunogen: Recombinant fusion protein of human Kaiso/Kaiso/ZBTB33(NP_006768.1).

Modification: Unmodified

Conjugate: Unconjugated