Product Description

KAP1 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB11137-50, MB11137-100

Product: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA

Swiss-Prot: Q13263

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-F, IHC-P, ICC/IF

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200

Background: Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 89 kDa; Observed MW: 100 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of KAP1 in K562, C6, 3T3, Hela lysates using KAP1 antibody., Immunocytochemistry analysis of KAP1 in Hela using KAP1 antibody, and DAPI, Immunohistochemistry analysis of paraffin-embedded Human lung cancer using KAP1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: TRIM28; KAP1; RNF96; TIF1B; Transcription intermediary factor 1-beta; TIF1-beta; E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripart

Immunogen: A synthetic peptide of human KAP1

Conjugate: Unconjugated

Modification: Unmodified