Product Description

KAP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90758-50, BS90758-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13263(Human) Q62318(Mouse) O08629(Rat)

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, ICC/IF, IHC, FC

All Applications: WB:1:2,000-1:5,000
ICC:1:50-1:200
IHC:1:50-1:200
FC:1:50-1:100

Background: TIF1β, for transcriptional intermediary factor 1-beta, also designated KAP1(for KRAB-associated protein 1), TF1β and TRIM28 (for tripartif motif-containing 28), is a member of the tripartif motif family characterized by three zinc-binding domains, a RING finger, B-boxes and a coiled-coil domain. Like TIF1α, TIF1β contains both a Cys/His PHD (plant homeodomain) finger and bromodomain that form a cooperative unit required for transcriptional repression. TIF1β mediates transcriptional control by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors and by binding DNA through its zinc finger. The human TIF1β gene maps to human chromosome 19q13.4 and encodes an 835 amino acid nuclear protein.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: KAP1 polyclonal antibody detects endogenous levels of KAP1 protein.

Bioworld Molecular Weight: 110 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of KAP1 on different lysates using anti-KAP1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: Hela, ICC staining KAP1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: TIF1-beta, KAP-1, KRIP-1, TRIM28, E3 SUMO protein ligase TRIM28, E3 SUMO-protein ligase TRIM28, FLJ29029, KAP 1, KAP-1, KRAB associated protein 1, KRAB interacting protein 1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP 1, KRIP-1, KRIP1, Nuclear corepressor KAP 1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1-beta, TIF1B, TIF1B, Transcription intermediary factor 1 beta, Transcription intermediary factor 1-beta, Trim28, Tripartite motif containing 28, tripartite motif containing protein 28, Tripartite motif-containing protein 28,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodified