Product Description

KAP1/TRIM28 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70504-50, BS70504-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q13263

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC, IP,

All Applications: WB,1:1000 - 1:3000 | IHC,1:100 - 1:200 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:200

Background: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 120kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using KAP1/KAP1/TRIM28 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunohistochemistry of paraffin-embedded human breast cancer using KAP1/KAP1/TRIM28 Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human esophageal cancer using KAP1/KAP1/TRIM28 Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded mouse spinal cord using KAP1/KAP1/TRIM28 Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: KAP1; PPP1R157; RNF96; TF1B; TIF1B; TRIM28

Immunogen: Recombinant fusion protein of human KAP1/KAP1/TRIM28(NP_005753.1).

Conjugate: Unconjugated

Modification: Unmodified