Product Description

[KD Validated] eIF4E Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80390-50, BS80390-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P06730

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IHC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IHC,1:50 - 1:200

Background: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 29KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry of paraffin-embedded Rat testis using EIF4E antibody at dilution of 0.0763888888888889. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Western blot analysis of extracts from wild type and eIF4E knockdown 293T cells, using eIF4E antibody at 1:578 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunohistochemistry of paraffin-embedded Rat testis using EIF4E antibody at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded Mouse liver using EIF4E antibody at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Alternative Name: AUTS19; CBP; EIF4E1; EIF4EL1; EIF4F; eIF-4E; EIF4E

Immunogen: Recombinant fusion protein of human eIF4EeIF4E.

Conjugate: Unconjugated

Modification: Unmodified