Product Description

KDM5A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90765-50, BS90765-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P29375(Human)

Host: Rabbit

Reactivity: Human

Applications: WB, ICC IF FC

All Applications: WB:1:500
ICC:1:50-1:400
FC:1:50-1:100

Background: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. Regulates specific gene transcription through DNA-binding on 5'-CCGCCC-3' motif. May stimulate transcription mediated by nuclear receptors. Involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Plays a role in the regulation of the circadian rhythm and in maintaining the normal periodicity of the circadian clock. In a histone demethylase-independent manner, acts as a coactivator of the CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER1/2 and other clock-controlled genes and increases histone acetylation at PER1/2 promoters by inhibiting the activity of HDAC1. Seems to act as a transcriptional corepressor for some genes such as MT1F and to favor the proliferation of cancer cells.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: KDM5A polyclonal antibody detects endogenous levels of KDM5A protein.

Bioworld Molecular Weight: 192 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of KDM5A on A549 cell using anti-KDM5A antibody at 1/500 dilution., ICC staining KDM5A in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name: RBBP-2, KDM5A, JARID1A, RBBP2, RBP2, Histone demethylase JARID1A, JARID1A, Jumonji/ARID domain containing protein 1A, Jumonji/ARID domain-containing protein 1A, Kdm5a, KDM5A, Lysine-specific demethylase 5A, RBBP-2, RBBP2, RBP2, Retinoblastoma binding protein 2, Retinoblastoma-binding protein 2 antibody

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified