Product Description

KDM6A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS71748-50, BS71748-100

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200

Background: This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Extra Notes: Western blot analysis of various lysates, using KDM6A antibody at 1:790 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit. Exposure time: 60s., Immunohistochemistry of paraffin-embedded human breast cancer using Kdm6a antibody at dilution of 1:200. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Bioworld Molecular Weight: 180KDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: KDM6A;KABUK2;UTX;bA386N14.2

Immunogen: Recombinant fusion protein of mouse KDM6A(NP_033509.1).

Conjugate: Unconjugated

Modification: Unmodified