Product Description

Ki67 Monoclonal Antibody

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Catalogue Numbers: MB66989-50, MB66989-100

Sizes: 50µl, 100µl

Swiss-Prot: P46013

Host: Mouse

Reactivity: Human

Applications: IHC

Background: Ki-67, named after the location where it was discovered (Kiel University, Germany), is a nuclear nonhistone protein that is universally expressed among proliferating cells and absent in quiescent cells . Ki-67 detects proliferating cells in G1, S, G2, and mitosis, but not in the G0 resting phase. Research studies have shown that high levels of Ki-67 are associated with poorer breast cancer survival . Research studies have explored the use of Ki-67, along with other markers, as potential prognostic or predictive markers in breast cancer and other malignant diseases .

Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification and Purity: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of Ki67 protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Ki67 expression in whole cell lysates., Immunohistochemical analysis of Ki67 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Bioworld Molecular Weight: ~ 358 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Antigen KI-67

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Ki67. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified