Product Description

KIM1 Rabbit Monoclonal Antibody

Catalogue Numbers: MB66410-50, MB66410-100

Sizes: 50µl, 100µl

Product: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.

Swiss-Prot: Q96D42

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB (1/500 - 1/1000)

Background: T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-1 (HAVCR1 (hepatitis A virus cellular receptor 1), KIM-1 (kidney injury molecule-1) was originally identified as a receptor for hepatitis A virus. TIM-1 also acts as a costimulatory receptor on T cells and following activation, associates with the TCR complex to upregulate signaling and cytokine production . Another TIM family member, TIM-4, is expressed by antigen presenting cells and is a ligand for TIM-1 . TIM-1 expressed by Th1 and Th17 cells was also recently shown to interact with P-selectin to mediate T cell trafficking during inflammation and autoimmune disease . NKT cells also express TIM-1, and engagement of TIM-1 on NKT cells leads to increased production of IL-4, but decreased production of IFN-gamma. TIM-1 is also a receptor for phosphatidylserine exposed by cells undergoing apoptosis. Detection of phosphatidylserine by TIM-1 expressed on NKT cells results in activation, proliferation, and cytokine production. Expression of TIM-1 on regulatory B cells is required for optimal production of IL-10. Mice lacking the TIM-1 mucin domain have decreased production of IL-10 by regulatory B cells, hyperactive T cells, increased levels of inflammatory cytokines, and enhanced severity of autoimmune disease. In addition, TIM-1 polymorphisms are associated with susceptibility to atopic diseases including asthma . Finally, expression of TIM-1 is increased in renal tubular epithelial cells following kidney injury.

Purification and Purity: The antibody was purified by immunogen affinity chromatography.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of KIM1 protein.

Extra Notes: Western blot analysis of KIM1 expression in A549 (A), MCF7 (B), U251 (C), HL60 (D), Raw264.7 (E), PC12 (F) whole cell lysates.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Name: KIM1; TIM1; TIMD1; Hepatitis A virus cellular receptor 1; HAVcr-1; Kidney injury molecule 1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T-cell immunoglobulin mucin receptor 1; TIM; TIM-1; T-cell membrane protein 1

Immunogen: A synthetic peptide of human TIM 1

Conjugate: Unconjugated

Modification: Unmodified