Kininogen 1 Polyclonal Antibody
Sizes: 50µl, 100µl
Catalogue Numbers: BS90771-50, BS90771-100
Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Swiss-Prot: P01042 Human
Host: Rabbit
Reactivity: Human, Mouse
Applications: WB, IHC
All Applications: WB:1:500-1:2,000
IHC:1:50-1:200
Background: (1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.
Purification and Purity: Protein A purified.
Storage and Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Specificity: Kininogen 1 polyclonal antibody detects endogenous levels of Kininogen 1 protein.
Bioworld Molecular Weight: Predicted molecular weight: 48, 72 kDa
Note: For research use only, not for use in diagnostic procedure.
Extra Notes: Western blot analysis of Kininogen 1 on mouse stomach lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature., Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Kininogen 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with ET7109-34 at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Alternative Name: HMWK, KNG1, BDK, KNG, Alpha-2-thiol proteinase inhibitor, BDK, BK, Bradykinin, Bradykinin included, Fitzgerald factor, FLAUJEAC FACTOR, High molecular weight kininogen, HMWK, Ile-Ser-Bradykinin, Kallidin I, Kallidin II, KNG, KNG1, KNG1, Low molecular weight growth-promoting factor, WILLIAMS FACTOR, Williams-Fitzgerald-Flaujeac factor antibody
Immunogen: Recombinant protein within human Kininogen 1 aa 500-644.
Conjugate: Unconjugated
Modification: Unmodified