Product Description

KIR3.4 Polyclonal Antibody

Catalogue Numbers: BS5775-50, BS5775-100

Sizes: 50µl, 100µl

Alternative Name: G protein-activated inward rectifier potassium channel 4; GIRK-4; GIRK4; Cardiac inward rectifier; CIR; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; IRK-4; KATP-1; IRK4; KATP1; Potassium channel, inwardly rectifying subfamily J member 5; KCNJ5;

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P48544

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: G protein-coupled inwardly rectifying potassium channels (Kir3.1 through Kir3.4) are coupled to numerous neurotransmitter receptors in the brain and are abundantly expressed in the olfactory bulb, hippocampus, neocortex, dentate gyrus, cerebellar cortex and thalamus regions of the brain. Also known as GIRK, Kir3 potassium channels localize to the soma and dendrites as well as axons of neurons. Liberated Gbg subunits from G protein heterotrimers bind to and regulate Kir3 channel activity. Gb3- and Gb4-containing Gbg dimers bind directly to cytoplasmic domains of Kir3 proteins and increase the K+ current while Gb5-containing Gbg dimers inhibit Kir3 K+ current. Kir3 activity is also inhibited by tyrosine phosphorylation. Brain-derived neurotrophic factor activates receptor tyrosine kinase B, which then phosphorylates Kir3 tyrosine residues, effectively inactivating the Kir3 channels.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: KIR3.4 polyclonal antibody detects endogenous levels of KIR3.4 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of KIR3.4 polyclonal antibody at 1:500 dilution
Lane 1: MCF-7 cell lysate
Lane 2: Raw264.7 cell lysate
Lane 3: PC12 cell lysate

Immunogen: Synthetic peptide, corresponding to the 374-419 of Human KIR3.4.

Conjugate: Unconjugated

Modification: Unmodified