Product Description

Kir6.2 (Phospho-T224) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS64536-50, BS64536-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q14654

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB:1:500~1:1000 IHC:1:50~1:200 IF:1:50~1:200

Background: ATP-sensitive K+ channels play important roles in many cellular functions by coupling cell metabolism to electrical activity. KIR6.1 and KIR6.2 are members of the KIR (inwardly rectifying potassium channel) family of potassium channels. Inward rectifying K+ channels possess a greater tendency to allow potasium to flow into the cell rather than out of it. These channels comprise two subunits: a KIR6.0 subfamily component and a SUR component, which is a member of the ATP-binding cassette protein superfamily. Mutations in the gene coding for these channels are a cause of an autosomal recessive disorder characterized by unregulated insulin secretion. The amino-terminal and carboxyl-terminal domains of KIR channel subunits are both intracellular, and the two intracellular domains of KIR6.2 physically interact with each other.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Kir6.2 (Phospho-T224) polyclonal antibody detects endogenous levels of Kir6.2 protein only when phosphorylated at Thr224.

Bioworld Molecular Weight: ~ 44 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Kir6.2 (Phospho-T224) polyclonal antibody at 1:500 dilution
Lane A: MCF-7 whole cell lysate
Lane B: The Liver tissue lysate of Mouse
Lane C: The Liver tissue lysate of Rat

Alternative Name: ATP-sensitive inward rectifier potassium channel 11; IKATP; Inward rectifier K(+) channel Kir6.2; Potassium channel, inwardly rectifying subfamily J member 11; KCNJ11

Immunogen: Synthetic phosphopeptide derived from human Kir6.2 around the phosphorylation site of Threonine 224.

Conjugate: Unconjugated

Modification: Phosphorylation