Product Description

KISS1R Polyclonal Antibody

Catalogue Numbers: BS60624-50, BS60624-100

Sizes: 50µl, 100µl

Alternative Name: KiSS-1 receptor; KiSS-1R; G-protein coupled receptor 54; G-protein coupled receptor OT7T175; hOT7T175; Hypogonadotropin-1; Kisspeptins receptor; Metastin receptor; KISS1R; AXOR12; GPR54

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q969F8

Host: Rabbit

Reactivity: Human

Applications: WB

All Applications: WB: 1:500~1:1000

Background: G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors and heptahelical receptors, are a protein family which interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers such as diacylglycerol, cyclic AMP, inositol phosphates, and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. G protein receptor 54 (GPR54), a member of the rhodopsin family of GCPRs, is the receptor for the Kiss1 gene product, metastin. Mutations in GPCR54 are associated with a lack of puberty onset and autosomal recessive idiopathic hypogonadotropic hypogonadism, a deficient or decreased function of the gonads. Proper function of GRP54 is essential for puberty. In the rat, GRP54 is expressed in the liver, intestine and most areas of the brain, while in the human it is expressed in the placenta, pituitary, pancreas and spinal cord.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: KISS1R polyclonal antibody detects endogenous levels of KISS1R protein.

Bioworld Molecular Weight: ~ 43 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of KISS1R polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: A549 whole cell lysate

Immunogen: A synthetic peptide corresponding to residues in Human KISS1R

Conjugate: Unconjugated

Modification: Unmodified