Product Description

KMT5A Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS90775-50, BS90775-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9NQR1(Human) Q2YDW7(Mouse)

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, FC

All Applications: WB:1:500-1:2,000
FC:1:50-1:100

Background: Protein-lysine N-methyltransferase that monomethylates both histones and non-histone proteins. All monomethylates 'Lys-20' of histone H4 (H4K20me1). H4K20me1 is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression. Mainly functions in euchromatin regions , benef playing a central role in the silencing of euchromatic genes. Required for cell proliferation, probably by contribute to the maintenance of proper higher order structure of DNA during mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substrate compared to free histones. Mediates monomethylation of p53 / TP53 at 'Lys-382', leading to repress p53 / TP53-target genes.

Purification and Purity: ProA affinity purified

Storage and Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: KMT5A polyclonal antibody detects endogenous levels of KMT5A protein.

Immunogen: Recombinant protein

Conjugate: Unconjugated

Modification: Unmodified

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of KMT5A on different lysates using anti-KMT5A antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: Mouse liver Lane 3: 293T Lane 4: 293, Flow cytometric analysis of 293T cells with KMT5A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).