[KO Validated] BRG1/SMARCA4 Polyclonal Antibody-BS74306

[KO Validated] BRG1/SMARCA4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS74306-50, BS74306-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51532

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:20 - 1:50

Background: The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 180kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using BRG1/BRG1/SMARCA4 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Western blot analysis of extracts from normal and BRG1/BRG1/SMARCA4 knockout 293T cells, using BRG1/BRG1/SMARCA4 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of HeLa cells using BRG1/BRG1/SMARCA4 antibody.

Alternative Name: BAF190; BAF190A; BRG1; CSS4; MRD16; RTPS2; SNF2; SNF2L4; SNF2LB; SWI2; hSNF2b; SMARCA4

Immunogen: A synthetic peptide of human BRG1/BRG1/SMARCA4(NP_003063.2).

Conjugate: Unconjugated

Modification: Unmodified