Product Description

[KO Validated] CDK4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80289-50, BS80289-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11802

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IP, , IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:100 | IP,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 35KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CDK4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Western blot analysis of extracts of 293T cells, using CDK4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Western blot analysis of extracts from wild type and CDK4 knockout HeLa cells, using CDK4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunohistochemistry of paraffin-embedded human colon using CDK4 antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Alternative Name: CDK4; CMM3; PSK-J3; cyclin-dependent kinase 4

Immunogen: A synthetic peptide of human CDK4(NP_000066.1).

Conjugate: Unconjugated

Modification: Unmodified