[KO Validated] Fatty Acid Synthase (FASN) Polyclonal Antibody-BS80301

[KO Validated] Fatty Acid Synthase (FASN) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS80301-50, BS80301-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49327

Host: Rabbit

Reactivity: Human, Rat

Applications: WB,

All Applications: WB,1:500 - 1:2000

Background: The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 273KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using Fatty Acid Synthase Rabbit pAb antibody at 1:1330 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.5s., Western blot analysis of Rat liver, using Fatty Acid Synthase Rabbit pAb antibody at 1:1330 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.5s., Western blot analysis of extracts from wild type and Fatty Acid Synthase Rabbit pAb knockout HeLa cells, using Fatty Acid Synthase Rabbit pAb antibody at 1:1330 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 0.5s.

Alternative Name: FASN; FAS; OA-519; SDR27X1; fatty acid synthase

Immunogen: Recombinant fusion protein of human Fatty Acid Synthase (FASN)(NP_004095.4).

Conjugate: Unconjugated

Modification: Unmodified