Product Description

[KO Validated] Hsp90β Polyclonal Antibody

Sizes: 50 µl, 100 µl

Catalogue Numbers: BS6142-50, BS6142-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P08238

Host: Rabbit

Reactivity: Human, Mouse, Rat, Monkey

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts from normal and Hsp90β knockout HeLa cells, using Hsp90β antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Bioworld Molecular Weight: 80kDa

Specificity: Polyclonal Antibodies

Alternative Name: D6S182; HSP84; HSP90B; HSPC2; HSPCB; Hsp90 beta; HSP90AB1

Immunogen: Recombinant fusion protein of human Hsp90β(NP_031381.2).

Conjugate: Unconjugated

Modification: Unmodified