[KO Validated] IDH1 Polyclonal Antibody-BS6918

[KO Validated] IDH1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6918-50, BS6918-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O75874

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200 | IP,1:50 - 1:100

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 46KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts from normal and IDH1 knockout 293T cells, using IDH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s., Immunofluorescence analysis of C6 cells using IDH1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of L929 cells using IDH1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IDH1 antibody. Western blot was performed from the immunoprecipitate using IDH1 antibody at a dilution of 1:1000.

Alternative Name: IDH1; HEL-216; HEL-S-26; IDCD; IDH; IDP; IDPC; PICD

Immunogen: Recombinant fusion protein of human IDH1(NP_005887.2).

Conjugate: Unconjugated

Modification: Unmodified